Supercritical CO2 extraction of hemp (Cannabis sativa L.)

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In this work, hemp (Cannabis sativa L.) seed oil was produced by extraction with supercritical CO2 under different extraction conditions (temperature, pressure and time). The objective was to evaluate the influence of extraction conditions on concentration of tocopherols, fatty acids and pigments in hemp seed oil.

The composition of hemp seed oil obtained with supercritical CO2 was compared with the hemp oil
extracted by n-hexane using Soxhlet method and with oil obtained by pressing using screw expeller.
Using supercritical CO2 extraction the extracts higher in concentration of tocopherol were produced.
The amount of – tocopherol in supercritical extracts ranged from 37.09 to 110.61 mg L−1, depending
on the applied process conditions, while -tocopherol content was significantly higher (2–3 times). The
content of pigments in the hemp oil obtained by supercritical CO2 had been changed significantly during the extraction time from 9.79 to 178.76 mg kg−1 for total chlorophyll content and 8.15 to 57.66 mg kg−1 for total carotene content. By selecting the relevant process conditions of supercritical extraction it is possible to obtain hemp seed oil with physical or nutritional properties of interest to the food industry.

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