Quantitative Analysis of Cannabinoids using the LCMS-8040 Triple Quad MS

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Evaluation and quantitation of a variety of cannabinoids on an LCMS-8040 triple quadrupole mass spectrometer.

Cannabichromene (CBC), cannabigerol (CBG) and ∆9-THC Acid A (THCA) standards were
purchased from Restek (Bellefonte, PA). Cannabinol (CBN), ∆8-THC (d8-THC), ∆9-THC (d9-
THC), ∆9-THC-D3 (d9-THC-D3) and Cannabidiol (CBD) were purchased from Cerilliant (Round
Rock, TX). A stock concentration of 1 µg/mL for each standard was used for MRM optimization.

The standards were combined into one solution and then serially diluted with 70/30 (%) methanol/water using 2-fold dilutions yielding concentrations ranging from 8192 ng/mL to 1
ng/mL. The IS (d9-THC-D3) was added to all levels. The standards (Figure 1) were transferred to autosampler vials and injected into a Nexera-LCMS-8040 system for analysis.

A Thermo Hypersil Gold (1.9 µm x 2.1 mm x 100 mm) was used with a binary gradient of 5 mM
ammonium acetate in water and acetonitrile.


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The gradient conditions are shown in Figure 2.

The flow rate was 0.5 mL/min with a run time of 6.8 min. The column temperature was 45º C
and the injection volume was 5 µL.




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