Scientists of the laboratory of phytochemistry of the Siberian Botanical Garden of Tomsk State University together with colleagues from the Bangkok University. Ramkhahanga conducted a study during which they developed a method for using ultrasound to increase the degree of extraction of biologically active substances (BAS) from plants, the press service of the TSU reports. In addition, during the experiments, biologists succeeded in discovering a new source of ecdysteroids (molting hormones) possessing adaptogenic, immunostimulating, hepatoprotective activity and capable of inhibiting the growth of sarcoma cells. The findings are published in the journal International Journal of Food and Biosystems Engineering.
Ecdysteroids are the molting hormones and metamorphosis of insects and crustaceans. However, animals themselves can not synthesize these substances and consume them from plants. The laboratory of phytochemistry of SibBS TSU is one of the few in the world that has been studying ecdysteroids for 30 years and searching for plants that they are part of. These secondary metabolites are very promising for pharmacology, on their basis, substances of different biological activity have been created abroad.
“Together with Thai colleagues, we studied three types of serpents. A chromatographic analysis of the seeds obtained from the Jena Botanical Garden (Germany) was preliminarily carried out. His results confirmed the high probability of the presence of ecdysteroids in adult plants, “says Larisa Zibareva, head of the laboratory of phyto-chemistry at TSU. “After that, these species were grown in the Botanical Garden of TSU, the raw materials were tested for the content of ecdysteroids and flavonoids using high-performance liquid chromatography and used to isolate pure compounds.”
The traditional method of extracting BAS involves five steps and takes an average of 12 hours. In the course of experiments, scientists used ultrasound of high intensity, which made it possible to accelerate the extraction process and reduce the time costs to one hour, that is, 12 times. Moreover, the yield of nutrients has increased.
The final part of the research was carried out at the University. Ramkhamenga in Bangkok. From Tomsk, individual biologically active sulfuric compounds were brought there to study chemical structures using NMR and mass spectrometry methods. This made it possible to identify the isolated substances and discover a new source of BAS – Serratula cupuliformis, a traditional plant in the northern part of China, in which ecdysteroids were first identified.
“The data obtained are not only of fundamental importance, they can find a successful application in pharmacology,” says Larisa Zibareva. – Based on steroid substances of vegetable origin, you can develop drugs that will be no less effective than synthetic “chemistry”, but at the same time they are safe for people. “
Let’s add that scientists of the phytochemistry laboratory intend to expand their research, in particular, to begin work with experts in the field of IT on computer prediction of biological activity of plants on the structure of secondary metabolite compounds.
The full text of an article published in the International Journal of Food and Biosystems Engineering .
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